Journal: World Journal of Gastrointestinal Oncology
Article Title: Pancreatic neuroendocrine microtumors in the elderly: A retrospective study using cadaveric pancreatic tissue
doi: 10.4251/wjgo.v17.i12.113198
Figure Lengend Snippet: Histopathological changes of pancreatic neuroendocrine microtumors in Case 4. A: Hematoxylin and eosin staining of pancreatic neuroendocrine microtumors (PNEMTs) of Case 4; B: Immunostaining of PNEMTs in A with synaptophysin (Syn) antibody; C: Immunofluorescence staining of PNEMTs with glucagon (GLU) antibody; D: GLU (green) with insulin (red) double immunofluorescence staining. White asterisk in A: The tumors exhibited an organoid-like architecture, with tumor cells arranged in a trabecular pattern; black asterisk in A: The surrounding pancreatic parenchyma appeared largely normal. Black arrows in A: Indicate a slight membrane structure between the PNEMTs and the pancreatic tissue. Black arrowheads in A: Acinar ductal metaplasia; White arrows in C: GLU-positive cells in the islets of Langerhans; Black and white arrows in D: Insulin-positive cells in PNEMTs and islets of Langerhans, respectively. GLU: Glucagon; Syn: Synaptophysin; Ins: Insulin.
Article Snippet: The primary antibodies used were polyclonal guinea pig anti-human insulin antibody (ready-to-use, IR00261-2J; Dako, Tokyo, Japan), rabbit anti-GLU antibody (1:100, ab92517; Abcam, Tokyo, Japan), mouse anti-human chromogranin A antibody (1:1000, AMAb90525; Atlas Antibodies AB, Stockholm, Sweden), rabbit anti-human synaptophysin (Syn) antibody (1:100, HPA002858; Atlas Antibodies AB, Stockholm, Sweden), and rabbit anti-Ki-67 antibody (1:100, ab16667; Abcam, Tokyo, Japan) diluted in PBS supplemented with 3% bovine serum albumin.
Techniques: Staining, Immunostaining, Immunofluorescence, Double Immunofluorescence Staining, Membrane
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